334 



BACTERIOLOGY 



3. Microspira luminosa (Beijerinck) 



Photobact. luminosum Beijerinck: Archives Neelandaises, XXIII, 1889, 104. 

 Morphology. Bacilli 0.6 : 2.0 /A, bent curved rods threads, variable. 

 Gelatin stab. Liquefaction rapid ; in later generations it occurs more slowly. 



Grow best in 3.5 per cent NaCl gelatin. Phosphorescence weak, silvery ; 



does not appear on meat and potato cultures, evident in sea water, on 



fish, and in 3.5 per cent NaCl sugar-free gelatin. 

 The last three species are doubtfully placed in this genus. 



4. Microspira Metschnikovi (Gamalei'a) Migula 



Vibrio Metschnikovi Gamaleia: Annales Pasteur Institut, 1888, 482. 

 Msp. Metschnikovi Migula: Die Natiirlichen Pflanzenfam., ^892, 33. 



Morphology. Bacilli like Msp. comma, but somewhat shorter and thicker. 

 A long spiral polar flagellum. 



Gelatin colonies. Quite like Msp. comma. 



Gelatin stab. Liquefied more rapidly than Msp. comma, but otherwise 

 identical. 



Bouillon. Strongly turbid ; a strong, white membrane on the surface ; in 24 

 hours a strong nitro-indol reaction. 



Potato. Slight growth at 20 ; at 37 a delicate brownish layer. 



Pathogenesis . Pigeons inoculated into the pectoral muscle die in 24 hours. 

 Muscles greatly swollen and yellowish as if cooked, and infiltrated with a 

 serous fluid containing many bacilli ; numerous bacilli in the blood and 

 organs. Intestines pale and filled with a grayish yellow fluid, with only 

 'a few bacilli present. Subcutaneous inoculations of guinea pigs cause 

 death in 24 hours. There is great bloody oedema with bacilli in the heart 

 blood. 



Habitat. Isolated by Gamalei'a from an epizootic of fowl, and by Pfuhl 

 (Zeitsch. f. Hygiene, 1894) from water. 



5. Microspira Schuylkilliensis (Abbott) 



Vibrio Schuylkilliensis Abbott: Jour. Expt. Med., 1896, I, No. 3. 



Morphology. Bacilli in fresh cultures, rather plump commas, often with a 

 decided curve, or nearly straight, ends rounded slightly pointed, long 

 spiral filaments uncommon, involution forms in old cultures. A single < 

 polar flagellum. Spores absent. Stain irregularly; not stained by 

 Gram's method. 



Gelatin colonies. Round, sharply defined, slightly granular, with fine irregular 

 lining as if creased, or concentric becoming moruloid, or again distinctly 



