342 



BACTERIOLOGY 



Potato. Growth yellowish white. 



Glucose bouillon. No gas. 



Milk. Not coagulated. Non-pathogenic. Strongly aerobic. 



Habitat. Soil. 



ADDENDA 



20. Microspira marina (Russell) 



B. marinus Russell: Zeitsch. f. Hygiene, XI, 1891, 198. 



Morphology. Bacilli small, straight curved spirals. Motion rotatory and 



progressive. 

 Gelatin colonies. Small, radially striped, becoming liquefied and rougher, 



with flocculi in the liquefied gelatin. 

 Gelatin stab. A rapid liquefaction, the liquid gelatin turbid, and a thin 



pellicle on the surface. 



Agar slant. A moist whitish expansion, like pus. 

 Potato. Growth thick, waxy, spreading. 

 Bouillon, with sea water, turbid, with a smooth white pellicle. 

 Habitat. Isolated from sea water and sea mud. 



SPIRILLUM Ehrenberg 



Spirally curved or corkscrew forms of variable thickness. Endospores present 

 in a few s'pecies. Cells actively motile, and possess at one or both poles 

 bundles containing four or more flagella. 

 I. Cell contents colorless EUSPIRILLUM of Migula. 

 A. Grow in nutrient gelatin or other culture media. 



i. Aerobic, i.e. grow in the presence of atmospheric oxygen. 



a. Non-chromogenic ; do not produce pigment on gelatin or agar. 

 * Cell plasma contains numerous black granules, very large spi- 

 rilla, 1.5-2.0 ft. broad and 20-30 /x long. 



1. Spirillum volutans Ehrenberg. 



** Cell plasma does not contain dark granules. Spirilla smaller, 

 f Gelatin liquefied, sometimes very slowly. 



Pathogenic to pigeons and guinea pigs. Comma-like. 



2. Spirillum Massauah Kruse. 



Terrestrial forms probably not pathogenic. 



3. Spirillum serpens (Miiller) Winter. 



4. Spirillum tenerrimum Lehmann-Neumann. 



