34 8 BACTERIOLOGY 



Boiiillon. Turbid ; bacilli very actively motile, 1-6 : 0.3 /* ; a scum on the 



surface. 

 Gelatin colonies. Very small, white ; do not increase in size ; borders erose, 



granulose ; no liquefaction. 

 Gelatin slant. A thin thread-like growth, 0.5 mm. wide; here the bacilli are 



undulate with spindle-shaped thickenings. 



Agar slant. In 24 hours at 37 a delicate partly transparent whitish growth. 

 Blood serum. Growth as on gelatin. 

 Lactose-litmus agar. Color unchanged. 

 Potato. At first a watery growth which disappears later. Grows in an 



atmosphere of hydrogen in absence of oxygen. 



Milk. Not coagulated. Indol produced. Gas in glucose bouillon at 37. 

 Pathogenesis . Subcutaneous inoculation of rabbits with 0.2-0.5 cc - of a 



bouillon culture causes a progressive elevation of temperature, followed 



after 5-10 days by a fall to normal. Similar inoculations of 0.1-0.2 cc. 



into man causes a chill and elevation of temperature. Bacilli found in 



the blood. 



3. Spirochaeta anserina Sakharoff 



Centralblatt f. Bakteriol.,XI, 1892, 203. 



Morphology. Resembles No. I. Has never been cultivated in artificial 



media. 

 Habitat. From the blood of geese in septicaemia. Inoculations into geese 



positive. 



II. NON-PATHOGENIC SPECIES. 

 4. Spirochaeta plicatilis Ehrenberg 



Abhandl. Berlin Akad., 1833, 313. 



Morphology. Bacilli very thin flexible filaments, 0.5:100-200 /*, ends 



rounded, undulations close and regular. 

 Habitat. Stagnant water containing decomposing vegetable matter. 



5. Spirochaeta dentium Cohn 



Beitrage Biol., I, Heft II, 180, Heft III, 197, 1875. 



Morphology. Bacilli long flexible spiral filaments of unequal thickness, and 

 irregular spiral windings 8-25 /x, long. Do not grow on culture media as 

 far as known. 



Habitat. From the mouth of healthy individuals. 



