THE CLASSIFICATION OF BACTERIA 353 



6. Mycobact. murisepticum (Fltigge) 



B. murisepticus Fliigge : Die Mikroorganismen, 1886. 



B. Septikamie bei Mdusen Koch : Aetiol. Wundinfectionsk., 1878. 



B. marinus Schroter: Kryptogamenflora Schlesien, III, 1886, 162. 



Probably identical with the preceding. 



7. Mycobact. malei (Loffler) Migula 



B. mallei Loffler : Arbeiten Kaiserlich. Gesundheitsamte, I, 1886, 141. 



Morphology. Bacilli small, slender, bent, 0.25-0.4:1.5-3 /x; may occur as 

 coccoid elements. According to Marx (Centralblatt f. Bakteriol., XXV, 

 274), may show diphtheria-like and branched forms. Stain badly. Grow 

 best on glycerin agar ; a scanty growth on blood serum. 



Agar slant. In 24-48 hours at 37 C. whitish translucent watery colonies. 



Gelatin colonies. After weeks the gelatin begins to soften and small funnels 

 are formed. Grows with an acid reaction of the medium. 



Potato. Growth yellowish reddish brown ; show pleomorphic, anthrax- 

 like threads which become swollen involution forms. Indol doubtful. 



Pathogenesis. Rabbits but slightly affected, white and gray mice immune. 

 Intraperitoneal inoculations of 1-2 cc. into male guinea pigs cause death 

 in 12-15 days; testicles swollen and reddened, tubercles on tunica 

 vaginalis, suppurating organ contains the bacilli from which pure cultures 

 can be made. 



Habitat. Secretions and ulcers and tubercles in glanders in men, horses, 

 cats ; sheep, goats, dogs, and rarely swine ; cattle and birds immune. 



8. Mycobact. lactis 



A Bacillus resembling B. diphtheria found in milk and American cheese Park-Beebe- 

 Williams : Sci. Bull. No. 2, Health Dept., New York City, 1895. 



Morphology. Bacilli more or less regular in shape and size resembling 

 diphtheria, but slightly thicker and of more variable length. On Loffler's 

 blood serum bacilli like diphtheria, except slightly thicker and a little 

 more irregular. 



Gelatin colonies. In 24 hours minute, punctiform ; microscopically, round, 

 entire, sharp, yellow, granular. No liquefaction. 



Gelatin stab. In depth, growth finely granular, white ; on the surface, growth 

 abundant, light yellow, becoming deeper yellow. 



Agar colonies. In 24 hours at 20 small, punctiform, cream-colored, which 

 microscopically are coarsely granular, entire, grayish ; later the surface 

 colonies are a little larger and have a decided yellow color. 





