374 BACTERIOLOGY 



Gelatin colonies. Deep: round, reddish, granular, border slightly uneven,, 

 dense or densely floccose. Surface: in 4 days, round, with pale ill- 

 defined margins, about 2 mm., slightly sunken; microscopically, densely 

 floccose in centre, and of a reddish color, thinner and lighter in color 

 toward the border. 



Gelatin slant. A narrow dense reddish stripe, beneath which the gelatin is. 

 slowly liquefied. 



Gelatin stab. In depth, a slight growth; on the surface, growth round thin, 

 flat pinkish, below which is a slowly liquefying funnel. 



Acid gelatin. No growth. 



Agar slant. Growth thin, translucent, glistening, limited, slightly pinkish,, 

 usually of discrete colonies. 



Bouillon. Medium clear; no membrane, and with a pinkish sediment. 



Potato. Growth elevated, pink pale reddish, limited, rather rough. 



Litmus milk. No change in 2 weeks. 



Glucose gelatin stab. No growth in depth. 



Pepton rosolic acid. Unchanged. Indol slight. Grows at 36 C. 



Habitat. Water. 



Houston, 27th Report Loc. Gov. Board, England, Supplement, 1897-98, 289,, 

 notes a species of Cladothrix which liquefies gelatin and produces an; 

 orange-pink pigment. 



Habitat. Soil. 



4. Cladothrix dichotoma Cohn 



Beitrage Biol., I, 3, 1875, 185. . 



Filaments 0.4 /x thick, composed of rod-like segments surrounded by a delicate 



sheath.' Short motile gonidia are set free at the apex of a filament. 



Filaments may assume spiral forms and exhibit the false dichotomous 



branching. According to Mace (Compt. rend. CVI, 1888, 1622) the 



organism shows the following cultural characters : 

 Gelatin colonies. In 4-5 days, small yellowish dots; later a brownish button 



with a whitish bloom ; later depressed, due to a slow liquefaction of the 



surrounding gelatin ; medium stained brown. 

 Gelatin stab. On the surface growth thin, grayish ; gelatin slowly liquefied ; 



medium remains clear, but is stained a deep brown. 

 Agar slant. At 35, a thick glistening layer, very adherent, with often a 



whitish bloom. The agar is stained brown. 

 Bouillon. Whitish radiate flakes ; medium clear, but stained brown. All 



cultures have a strong mouldy odor. A species named Cl. dichotoma was 



isolated by Ravenel (I.e., 15), with the following characters: forms- 



