CYTOLOGY 



35 



Allow the chloroform gas to flow for a moment, in to the cell. 

 Note the effect of chloroform upon ciliary activity. 



(5) How many times may the cilia be narcotized with chloroform 

 and revived again through ventilation? 



(6) Repeat (4) with ether in place of chloroform. 



(7) Repeat (5) with ether in place of chloroform. 

 (c) Determine the action of alcohol vapor upon cilia. 



VI. TO DETERMINE THE AMOUNT OF WORK DONE BY CILIA. 



1. Appliances. Physiological operating case; frog board; cork 

 board 10 cm. long by 5 cm. wide; a centimetre rule; a block of wood 

 4 cm. or 5 cm. in height; a set of weights as follows: 50 mgm. and 

 100 mgm., 3 mm. square; also 100 mgm. and 200 mgm., 5 mm. 

 square. 



FIG. 12 



Apparatus for use in determining the amount of work done by cilia. (For description 



see text.) 



2. Preparation. Pith a frog and destroy cord. Dissect out 

 oesophagus and stomach as directed in Lesson IV. Fix to cork 

 board so that the long axis of the oesophagus shall be parallel with 

 the long axis of the board. 



3. Operation. Wash off ciliated surface, remove surplus moisture 

 with filter paper, and place a lead weight gently on the anterior end 

 of the oesophagus. 



