162 SPECIAL PHYSIOLOGY 



2. Preparation, (a) To Prepare Myosin. (1) Take one pound 

 of lean meat, grind it in the meat hasher; soak and wash repeatedly 

 until the tissue is nearly white and quite free from hsemoglobin. 



(2) Put the washed muscle tissue into a flask with an equal bulk 

 of a 20 per cent, solution of ammonium chloride; shake from time 

 to time for twenty-four hours. 



(3) Strain off the liquor and add it to twenty volumes of distilled 

 water. Myosin is precipitated. Wash the precipitate, redissolve 

 one-fourth of the precipitate in 10 per cent. NaCl and label : Saline 

 Solution of Myosin. 



(b) To Prepare Syntonin. To the remaining three-fourths of the 

 washed myosin add several volumes of 0.1 per cent, hydrochloric 

 acid. In a very short time the myosin will be dissolved and changed 

 to syntonin. 



(c) To Prepare Dilute Egg Albumin. Make an opening in end oi 

 the shell of an egg; drain off the white of the egg, catching it upon a 

 coarse linen cloth a towel serves the purpose well ; press the albumin 

 through the meshes of the linen into a beaker; add 400 or 500 c.c. oi 

 distilled water; transfer the mixture to a 1 -litre cylinder, and shake 

 vigorously; after a short time filter through pure absorbent cotton oi 

 strain through fine linen. 



(d) To Prepare Acid Albumin. To 100 c.c. of dilute egg albumin 

 add an equal quantity of 0.2 per cent, hydrochloric acid; place the 

 mixture in the incubator for two or three hours. Though the change 

 begins at once, it will probably not be complete before the time sug- 

 gested. If one wishes to isolate the acid albumin from the mixture, 

 he has only to carefully neutralize with sodic hydroxide, precipitating 

 the acid albumin, and to wash the precipitate with distilled water. 

 For the purposes for which it is to be used in the following demon- 

 stration, it may be left in the acid solution, which represents 0.1 per 

 cent. HC1. Label: Acid Albumin Solution in 0.1 per cent. HCl. 



(e) Make an aqueous solution of the commercial " peptone," and, 

 though -the peptone is present in small proportions, label it Pro- 

 teoses. 



(/) Make an aqueous solution of a few grams of Griibler's pure 

 peptone and label: Peptone. 



(g) Dissolve a few grams of gelatin in distilled water. 



(h) To Prepare Millon's Reagent. 1. To 100 grams of pure mer- 

 cury add an equal weight of concentrated nitric acid, c. p. The reac- 

 tion proceeds at room temperature, though gentle heat may be applied 

 to complete the solution of the mercury. 2. Cool the mixture; add 

 two volumes of water; after twelve hours decant the supernatant 

 liquid Millon's Reagent. 



3. Experiments and Observations. (1) The Heat Test. Pour 

 into test-tubes a few cubic centimetres of each of the following pro- 

 teid solutions and subject each in turn to a temperature of 63 C., and, 



