APPENDIX. 



1. NORMAL SALINE SOLUTION. 



This solution or, as it is also called, normal salt solution or physio- 

 logical salt solution, is so much used in the physiological laboratory 

 that it should be made in considerable quantity and always easily 

 accessible. 



FORMULA. 



Common salt (c. p.) 30 grams. 



Distilled water 5 litres. 



It is convenient to keep the solution in a siphon bottle. It is thus 

 protected from dust and evaporation, and is always easily accessible. 



2. FROG BOARDS. 



There is probably no more satisfactory or economical frog board 

 than a piece of dressed soft pine 15 cm. by 30 cm., and 1 or 2 cm. 

 in thickness. Some prefer to use cork board, which comes in pieces 

 10 cm. by 25 cm. and f cm. in thickness. In the case of either, two 

 or three coats of orange shellac should be given to the boards before 

 they are put into use. 



3. THE PHYSIOLOGICAL OPERATING CASE. 



A convenient case, and one which will be sufficient in the simple 

 experiments presented in this book, contains the following instru- 

 ments: one medium scalpel; one small scalpel with narrow blade; 

 one medium scissors ; one microscopic scissors ; one medium dissecting 

 forceps; one microscopic forceps with curved, serrated jaws; two 

 serre-fine forceps with stiff springs and serrated jaws; one grooved 

 director and aneurysm needle; one silver probe; one blunt needle 

 for pithing frogs, and two dissecting needles. 



The case may be of leather or leatherette. Such a case may be 

 used nearly as much in the histological as in the physiological labor- 

 atory. 



