iv THE BLOOD: FORMED CONSTITUENTS 121 



water 60 grms., acetic acid 3 grms. In 5-10 minutes the cover-glasses are taken 

 out of the haematoxylin, washed in water, and stained for the second time by 

 dipping them for a few moments into a 1 per cent solution of eosin. They 

 are then washed again in distilled water, wiped at the edges with filter-paper, 

 dried over the; flame, and mounted on the slide with a drop of Canada balsam 

 dissolved in xyloL 



The nuclei and blood-platelets stain blue with the haematoxylin, .the 

 protoplasm pink with the eosin. 



A copious literature has recently sprung up in regard to 

 flsmotic phenomena, and the resistance of the erythrocytes to 

 yielding their haemoglobin, when brought into salt solutions of 

 different concentrations. Since, however, this subject is intimately 

 connected with the physico-chemical structure of the blood 

 plasma, we shall consider it in the next chapter. 



The important question of the origin, formation, and 

 destruction of Erythrocytes and Leucocytes will be discussed in 

 treating of the function of the haematopoietic and haematolytic 

 organs. 



BIBLIOGRAPHY 



WELCKER. Zeitschr. f. rat. Med., 1858. 



PREYER. Die Blutkrystalle. Jena, 1871. 



A. ROLLETT. Hermann's Handbuch d. Physiol., 4, 1880. 



C. BIZZOZERO. Arch. It. Biol., 1882, 1883. 



HAYEM. Arch, de pliysiol., 1883. Gaz. med., 1883. 



HEDIN. Strass. Arch. f. Physiol., 1890. 



E. A. SCHAFER and A. GAMGEE. Schafer's Text-Book of Physiology, i. 1898. 

 H. F. HAMBURGER. Osmotischer Druck u. lonenlehre. Wiesbaden, 1901-5. 

 R. HOBER. Phys. Chemie der Zelle u. der Gewebe, 2nd ed. Leipzig, 1906. 



F. WEIDENREICH. Die roten Blutkbrperchen-Ergebnisse. Merkel and Bonnet, 



1903-4. 



GRAWITZ. Klin. Path, des Blutes, 1902. 

 ENGEL. Leitfaden z. klin. Unters. des Blutes, 1902. 

 FOA. Arch. d. scienze med. Turin, 1906. 



Recent English Literature : 



W. MYERS. The Causes of the Shape of Non-nucleated Red Blood Corpuscles. 



Journ. of Anat., xxxiv. 3, p. 351. 

 A. GAMGEE. On the Behaviour of Oxy haemoglobin, etc., etc., in the Magnetic 



Field. Proc. Roy. Soc., Ixviii. 450, p. 503. 

 J. HALDANE. The Colorimetric Determination of Haemoglobin. Journ. of 



Physiol., 1900-1, xxvi. 497. 



G. N. STEWART. The Conditions that underlie the Peculiarities in the Behaviour 



of the Coloured Blood Corpuscles to certain Substances. Journ. of Physiol., 



1900-1, xxvi. 470. 

 5. PESKIND. Notes on the Action of Acids and Acid Salts on Blood Corpuscles 



and some other Cells. Amer. Journ. of Physiol., 1903, viii. 99 and 404. 

 O. N. STEWART. The Behaviour of Nucleated Blood Corpuscles to certain 



Haemolytic Agents. Amer. Journ. of Physiol., 1903, viii. 103. 

 G. T. .KEMP. Relation of Blood Plates to the Increase in the Number of Red 



Corpuscles at High Altitudes. Proc. of the Amer. Physiol. Soc. (Amer. 



Journ. of Physiol.), 1902, vi. p. xi. 

 G. T. KEMP and 0. 0. STANLEY. Some- New Observations on Blood Plates. 



Proc. of the Amer. Physiol. Soc. (Amer. Journ. of Physiol.), 1902, vi. p. xi. 

 . C. GUTHRIE. The Laking of Dried Red Blood Corpuscles. Amer. Journ. of 



Physiol., 1903, viii. 441. 

 G. N. STEWART. The Influence of Cold on the Action of some Haemolytic Agents. 



Amer. Journ. of Physiol., 1903, ix. 72. 



