APPENDIX 309 



Benedict's Solution for Carbohydrate Estimation. 

 See under description of the method in the laboratory direc- 

 tions. 



Congo Paper. 



Dissolve 1 gram of Congo red in 90 c.c. water, add 10 

 c.c. 95% alcohol. Dip filter paper into this solution, and 

 allow to dry. 



Esbach's Reagent. 



Dissolve 10 grams of picric acid and 20 grams of citric 

 acid in 1 liter of water. 



Fehling's Solution. (Quantitative.) 



Fehling's solution is composed of two definite solutions 

 a copper sulphate solution and an alkaline tartrate solution, 

 which may be prepared as follows: 



A. Copper sulphate solution = 34.65 grams of copper sul- 

 phate dissolved in water and made up to 500 c.c. 



B. Alkaline tartrate solution = 125 grams of potassium hy- 

 droxide and 173 grams of Rochelle salt dissolved in water 

 and made up to 500 c.c. 



These solutions should be preserved separately in rubber- 

 stoppered bottles and mixed in equal volumes when needed 

 for use. This is done to prevent deterioration. 



Only the copper salt need be weighed on the quantita- 

 tive balance. 



Fehling's Solution. (Qualitative.) 



Amounts as in quantitative, but weighed on the ordinary 

 balance. 



Folin-Schaffer Reagent. 



This reagent consists of 500 grams of ammonium sulphate, 

 5 grams of uranium acetate, and 60 c.c. of 10% acetic acid in 

 650 c.c. of distilled water. 



Formalin. (Neutral.) 



Dilute formalin 1-4 with distilled water, add a small 



