150 MICROBES, FERMENTS, AND MOULDS. 



mixed microbes, presenting no trace of the original 

 liquid, and this was done in vessels protected from air- 

 germs. These cultures may be carried to the eighth 

 generation. 



Asses and horses inoculated with liquid containing 

 the microbes produced by this culture have died with 

 the lesions characteristic of glanders (glanderous 

 tubercles in the spleen, lungs, etc.). Cats and other 

 animals which have been inoculated in the same way 

 die with glanderous tubercles in the lymphatic glands 

 and other organs. 



It follows from these experiments that the microbe 

 which causes this disease is always reproduced in the 

 different culture liquids with its characteristic form 

 and dimensions ; that uni-ungulates can be inoculated 

 with it, as well as man and other animals. In fact, this 

 microbe is the essential cause of the disease. 



VIII. PEBRINE AND FLACHERIE, DISEASES AFFECTING 

 SILKWORMS. 



We have already spoken of muscardine, a silk- 

 worm's disease produced by a microscopic fungus ; 

 two other diseases are caused by distinct microbes, of 

 which we must shortly speak. 



Pebrine. In the silkworm nurseries, in which this 

 disease prevails, the silkworms which issue from the 

 eggs, technically called seed, are slowly and irregularly 

 developed, so as to vary greatly in size. Many die 



