44 MICRO-ORGANISMS AND DISEASE. [CHAP. 



bacteria that do not grow in gelatine at the temperature at 

 which this latter remains solid, the same method of plate- 

 cultivation can be used, but substituting the gelatine by the 

 Agar-Agar mixture above mentioned, care must be taken 

 not to proceed with the inoculation of the Agar-Agar mixture 

 before the temperature has fallen to about 50 C. All other 

 manipulations remain the same. 



2. Inoculations with Blood, Juices ; and Tissues. To 

 establish a cultivation from blood of a dead animal, cut open 

 the thorax by removing the sternum with clean scissors, cut 

 open the pericardial sac, pierce with the pointed end of a 

 fresh capillary pipette the wall of the right ventricle or right 

 auricle, and allow a drop or two of blood to ascend into the 

 pipette, or if a larger quantity is required suck it up. With- 

 draw the pipette and inoculate new culture-tubes as above. 

 Or, if blood of a large vein is required, separate the vessel 

 with clean instruments, and make a small incision with clean 

 scissors and push the pointed end of the capillary pipette well 

 forward. If juice of a lymphatic gland, or spleen, or other 

 parenchymatous organ be required, pierce the organ after 

 having washed its surface with strong solution of perchloride of 

 mercury (Koch), with the pointed end of a capillary pipette, 

 then push it into the part required for a little distance, and 

 squeezing the organ press a drop or two of the juice into it. 

 The same procedure is adopted when the pus of an abscess 

 is required, the wall of which can be pierced with the pointed 

 end of the capillary pipette. If not, a slight incision is made 

 and the pipette introduced through this into the abscess. 

 If blood of a living animal is required, expose a vessel with 

 clean instruments, make a small incision with clean scissors, 

 push through this incision the pointed end of the capillary 

 pipette well forward, and allow the blood to rise into the 

 capillary tube. If blood of a living human being is required, 



