130 MICRO-ORGANISMS AND DISEASE. [CHAP. 



third day) there appeared on the surface of the inoculated 

 material the first traces of the growth in the form of minute 

 transparent droplets which consisted entirely of the 

 characteristic bacilli. Cultivating these through several 

 generations or transferences, and then inoculating with them 

 a horse, rabbits, guinea-pigs, and mice, positive results were 

 obtained, especially in the guinea-pigs, which appear very 

 susceptible to the disease. On the site of the subcutaneous 

 inoculation appears an ulcer with indurated base, speedily 

 enlarging and spreading ; other ulcers follow in the 



FIG. 62. Pus OF A PULMONARY ABSCESS IN A HORSE DEAD OF GLANDERS. 



1. The nuclei of pus cells. 



2. The glanders-bacilli. 



Magnifying power 700. (The preparation had been stained with methylene-blue.) 



neighbourhood, the neighbouring lymphatic glands become 

 swollen, and the general infection follows, in the form of 

 nodules and ulcers on the nasal septum, and nodules in the 

 internal organs. In the guinea-pig a characteristic tumour 

 of the testis, ovary, and vulva is often observed. In all 

 these cases the diseased tissues and organs contained the 

 characteristic bacilli. 1 



1 Other writers on the bacilli of glanders are Drs. Bouchard, Caoitan, 

 Charvin, in the Revue medicale jranfaise, Dec. 30, 1882. N. P. 

 Wassilieff, in Deutsche med. Woch. II, 1883, observed the bacilli in 

 human glanders. 



