1 64 MICRO-ORGANISMS AND DISEASE. [CHAP. 



hardened ; it is as follows : Take of a 2 per cent, watery 

 solution of gentian-violet 12 ccm., and of a saturated watery 

 solution of anilin oil 100 ccm. Mix. This is used like an 

 ordinary staining-fluid for the first stain. For the second or 

 contrast stain the following solution is used : 



Bismarck brown . . . . i gramme. 

 Spiritus vini rectificati (sp. gr. '830) . 10 ccm. 



Distilled water . . . . 100 ccm. 



The sections remain in a few drops of this solution for 

 fifteen minutes. This method yields the finest specimens of 



Fw. 89. FROM A PREPARATION OF CASEOUS MATTER FROM PULMONARY 

 DEPOSITS IN BOVINE TUBERCULOSIS, STAINED AS IN PRECEDING FIGURE. 



Magnifying power 700. 



tubercle-bacilli in sections through tuberculous growths that 

 I have seen ; unfortunately the colour of the bacilli is very 

 liable to fade. 



In the case of tuberculous sputum, or similar matter, a 

 small droplet or particle is spread out in a thin layer on the 

 cover-glass, well dried by passing it over the gas-flame of a 

 Bunsen burner, and then stained in the way described in 

 Chapter I. Sections of tubercles, fresh or hardened, are 

 stained without first drying. 



