148 



MICRO-ORGANISMS AND FERMENTATION. 



skin, partially covering the spores ; or it may gradually 

 dissolve during germination. 



Budding can occur at any point on the surface, of the swollen 

 spores ; this budding usually takes place after the wall of the 

 mother-cell has been ruptured or dissolved, but it also 

 occasionally takes place within the mother-cell. After the 



FIG. 40. Germination of the spores of Saccharornyces Ludwigii (after HANSEN) : 

 a c represent a gypsum-block culture 12 days old ; d h, a similar culture, one and- 

 a-half months old. 



buds have formed, the spores may remain connected, or they 

 may soon be detached from each other. 



An especially curious and exceptional case is that of the 

 wall separating two spores being dissolved, so that & fusion of 

 the spores results (see Fig. 39, e e"" and h h"). HANSEN 

 assumes that the biological significance of this phenomenon is 

 that the spores, placed under unfavourable conditions, have a 

 greater chance of forming buds. One spore plays the part of a 



