188 



MICRO-ORGANISMS AND FERMENTATION. 



Ascospore-formation (Figs. 37-39, 43, 1): 1 

 At 3 7 '5 C. no ascospores are developed. 



36-37 the first indications are seen after 29 hours. 



35 

 33-5 

 30 

 25 



25 



23 



20 



23 



27 



50 



65 



10 days. 



17-5 



16'5 



11-12 



9 no ascospores are developed. 



Spores strongly refractive to light. Wall of spores very 

 distinct. Size of spores 2*56 //. 



FIG. 48. Saccharomyces cerevisise I. (HANSEN). 

 (after HANSEN). 



Film-forms at 156 C. 



Film- formation : 

 At 38 C. no film-formation occurs. 



33-34 feebly-developed film-specks are 



seen after - 

 26-28 

 20-22 



} (Fig. 48) ( - 



no film-formation occurs. 



13-15 



6- 7 

 5 



9-18 days. 

 7-11 

 7-10 

 15-30 

 2 3 months. 



J The preparatory treatment of a Saccharomyces species for these investi- 

 gations must be made in the following manner: After the cells have been 

 cultivated for some time in ordinary wort (14 Ball.) at the ordinary room- 

 temperature, the vigorous young cells obtained are introduced into fresh wort, 

 in which they are allowed to develop for about twenty-four hours at 25 to 

 27 C. This growth is used for the gypsum-block culture. 



