ALCOHOLIC FERMENTS. 195 



opalescent appearance, the addition of Sacch. Pastorianus III. 

 will in certain cases effect a clarification. 



According to investigations made by the author, a strong 

 infection of low-fermentation yeast with this species may in 

 certain cases effect an excellent clarification and good "break- 

 ing" in both the fermentation vessel and in cask. 



Ascospore-formation (Fig. 43, 4): 

 At 29 C. no ascospores are developed. 



27-28 the first indications are seen after 35 hours. 



26'5 30 



25 28 



22 29 



17 44 



16 53 



10'5 7 days, 



o'o ,, y ., 

 4 no ascospores are developed. 



Size of the spores 2 5 /*. 

 Film-formation : 

 At 34 C. no film-formation occurs. 



26-28 feebly-developed film-specks are 



seen after - 7-10 days. 



20-22 9-12 



13-15 



6- 7 

 3- 5 



(Fig. 55) 



10-20 

 1 2 months. 

 5- 6 



2-3 no film-formation occurs. 



Microscopic appearance of the cells in the films : 



At 20-28 C. : Almost the same forms as in the sediment- 

 ary yeast. 



At 15-3 C. : Strongly-developed colonies of elongated, 

 sausage-shaped or thread-like cells, which closely resemble a 

 mycelium in appearance (Fig. 55). 



In old cultures of films, the cells have the same forms 

 as at 3-15* C., but are often still thinner and more thread- 

 like. 



Streak cultures of this species in gelatine yeast-water, after 



