474 BOARD OF AGRICULTURE. [Pub. Doc. 



with a culture made from the liver of the Princeton cow, in 

 bouillon, August 21. Four cubic centimeters of this cult- 

 ure were injected into the subcutaneous connective tissue 

 on the right shoulder. In twenty-four hours there was a 

 swellinof at the seat of inoculation the size of half a hen's 

 egg, which was hard and very painful on pressure. The 

 heifer's temperature arose to 103° F., where it remained 

 four or five days, when it gradually subsided to normal. 

 There was very little loss of appetite ; the animal fed spar- 

 ingly for two or three days, and then fed as usual. The 

 swelling remained for some time, becoming less painful, and 

 bad not entirely disappeared when she was disposed of three 

 weeks later. 



Whether this inoculation would protect the heifer if she 

 had been introduced into an infected pasture, is a question ; 

 it very probably would. Furthermore, would the germ in- 

 troduced into the subcutaneous tissue of the shoulder act in 

 all cases as it did with this animal, or, if tried on a larger 

 scale, would it act fatally in some cases? A single experi- 

 ment proves nothing, beyond suggesting the importance and 

 necessity of further study, and experimentation upon a suf- 

 ficiently large scale to lay a foundation upon which to base 

 correct conclusions. 



Kitt is quoted, in Freidberger and Frohner's " Pathology 

 and Therapeutics of the Domestic Animals," fourth Ger- 

 man edition, Vol. 2, page 416, as saying that animals 

 inoculated with cultures of the blackleg bacillus are given 

 immunity from natural infection ; ])ut it would require fur- 

 ther study of the behavior of the bacillus separated from the 

 Worcester County disease before it could be decided that 

 this was the case there. 



In the fifteenth annual report of the Bureau of Animal 

 Industry, 1898, there is a very interesting report upon black- 

 leg by Dr. Victor A. Norgaard, in which he gives an ac- 

 count of its history, geographical distribution, distribution 

 in the United States, symptoms, post-mortem appearances 

 and prevention, to which the reader is referred for more 

 detailed information. Norgaard says that in ninety-nine 

 per cent, of all the cases the tumors develop on the surface 

 of the body ; and this would seem to indicate that the 



