216 TECHNICAL BULLETIN U 



always produced a complete iiiiiimnity of a cutaneous nature after the de- 

 velopment of the local pox eruption. 



Additional experuncnts showed that this cutaneous vaccine always produced 

 the local pox eruption, which was essential to the development of a complete 

 immunity, but its administration was followed by a decrease in egg produc- 

 tion. It was also determined that com})lement-fixing antibodies were not 

 produced during the development of cutaneous immunity against contagious 

 epithelioma. 



A tentative standard for the cutaneous vaccine was adopted. The virus to 

 be used should have an incubation period of from four to seven days and, 

 therefore, must be less than one year old. The vaccine should contain 200 

 milligrams of such a virus suspended in 50 cc. of a 40 per cent glycerol- 

 physiological saline solution. The product shovdd not be attenuated by heat. 

 It should be used within 25 days after its manufacture, preferably within 10 

 to 15 days, because it does not always produce a complete immunity when 

 older. If continuoTi.'^ ice-box storage is not available, 0.5 per cent phenol 

 siiould lie added as a preservative. 



BIBLIOGRAPHY. 



(1) Beach, J. R. : The treatment and prevention of chicken-pox (con- 



tagious epithelioma) of fowls. Jour. Amer. Assoc. Instr. and Invest. 

 Poultry Husb., 1920, vii. No. 1. 



(2) Pyle, Normax J.: The therapeutic efficiency of avian diphtheria, rouj), 



and bird pox vaccines and bacterins. Mass. Agric. Exp. Sta. Tech. 

 Bull. 10, 1926. 



(3) Sweet, Clifford D.: A study of epithelioma contagiosum of the com- 



mon fowl. Calif. Univ. Pubs. Zool., 1913, ii, 29-51. 



(4) VAX Heelsbergex, T.: Vaccination against diphtheria and fowl po.\ 



with Antidiphtherin. Vet. Rec, 1925, v. No. 24. 



(5) HoL, G. H. G. : Antidiphtherin vaccine for diphtheria and pox in poul- 



try. North Amer. Vet., 1927, viii, 44-46. 



(6) Doyle, T. M.: Tests of the de Blieck-van Heelsbergen method of im- 



munization against fowl pox. Vet. Rec. 1926, vi, 741-743. 



(7) DE Blieck, L.: Diphtheria and pox in chickens, and the combating of 



this disease. Proc. World's Poultry Congres.s, Ottawa, Canada, 1927, 

 290-294. 

 (8j Paxisset, L. and "S'erge, J.: Immunity in a\ian diphtheria and con- 

 tagious epithelioma (trans, title). Compt. Rend. Acad. Sci. (Paris), 

 1923, clxxviii, 345-347. Cited in E. S. R., Aug. 1924, li, 184. 



(9) JoHxsox, W. T.: Fowl pox prevention by immunization. Jour. Amer. 



Vet. Med. Assoc, 1927, Ixxi, N. S. xxiv, 750-763. 



(10) GiLDows E. M.: Fowl pox. New Eng. Homestead, Jan. 21, 1928, xcvi, 



14-15. 



(11) Verge, Jeax: Reclierches Experimentales sur I'Affection Diphtero- 



Variolique des Oiseaux. 1926. 230 p. Toulouse, France, J. Bonnet. 



(12) Beach, J. R.: The immunization of fowls against chicken-pox (Epith- 



elioma contagiosimi) by subcutaneous injection of virus. Hilgardia 

 (Calif. Agric. Exp. Sta.), 1927, iii, 41-97. 



(13) Besredka, a.: Local immunization. Edited and translated by Harry 



Plotz, 1927. 181p. Baltimore, U. S. A., AVilliams and Wilkins. 



