570 BOARD OF AGEICULTURE. [Pub. Doc. 



Along the lateral border of the right lung similar vascular fringes of 

 tissue containing tubercles. These fringes extend dorsad for about two 

 inches on ventral and cephalic lobes. 



Diffuse eruption of minute tubercles on pleural aspect of diaphragm, 

 right half 



Large caudal mediastinal gland contains large numbers of tubercles, 

 varying in size from mere points to those one-eightli inch in diameter, 

 and showing beginning necrosis. Left bronchial gland several times 

 normal size. Ventral mediastinal gland several times normal size, and 

 containing several large centrally caseoiis foci. One mesenteric gland 

 contains several small necrotic tubercles. 



In this case many of the bacilli had been deposited in the fleshy 

 portion of the diaphragm and some under the skin, and they were 

 thus prevented from exerting their greatest power ; nevertheless, 

 the lesions are quite severe. It is not improbable that this culture 

 would have been much more destructive had it been used six or 

 seven months earlier. 



Experiment III. — This comprised six head of cattle. The 

 details of the experiment do not differ from those of experiment 

 II. The animals had been tested with tuberculin under the direc- 

 tion of the Board. 



The cultures of tubercle bacilli used in these tests were from 

 cases of human and bovine disease, and comparatively fresh : — 



Sputum culture IV., six months, ten days old, from a case of phthisis 

 in Melrose, Mass. Patient was subsequentlj- reported recovered. 



Sputum culture V., one month, nineteen days old, from a case of 

 phthisis of about two years' standing (New Hampshire). 



Sputum culture VI., one month, twelve days old, from a case of 

 phthisis in Winthrop, Mass. 



Bovine culture III., four months, seven days old, from a cow with 

 advanced lesions of lungs and liver (probably from Carlisle, Mass.). 



Bovine culture IV., four months, three days old, from a cow with 

 slight disease of mediastinal glands. 



Bovine culture V., four months, three days old, from a cow with 

 moderate disease of the lungs and portal gland. 



This set of cultures is thus younger than the preceding set, with 

 odds in favor of two of tlie sputum cultures. The bovine cultures 

 were of nearly the same age. 



The animals at my disposal were, unfortunately, not of the 

 same age, and in assigning the cultures the advantage was given 

 to the human cultures : — 



Sputum IV., yearling, 525 pounds, no permanent incisor teeth. 

 Bovine III., yearling bull, 645 pounds, no permanent incisor teeth. 

 Sputum v., cow, 676 pounds, about four years old. 



