No. 4.] CATTLE COMMISSIONERS. 571 



Bovine IV., cow, 850 pounds, about twelve years old. 



Sputiuii VI., cow, 865 pounds, about three and one-half years old. 



Bovine V., cow, 875 pounds, about six years old. 



The injection of the cultures was carried out as in experiment 

 II., excepting that the point of insertion of the needle was chosen 

 higlier up, about eleven inches above the elbow of the animal 

 when in the standing position. The lengtli of the needles used 

 was about two inches. 



The care of the animals was the same as that bestowed on the 

 preceding lot, excepting that the bovine and the sputum animals 

 were kept separate in the out-door enclosure from the start, the 

 one lot being out in the morning, the other in the afternoon. 

 They were kept two months, with the exception of the young bull 

 (bovine culture III.), which died seventeen days after the inocu- 

 lation. The four cows of this lot gave at the start altogether 

 about eight quarts of milk. The amount slowly diminished, until 

 in the sixth week a very little, amounting perhaps to one quart in 

 two days, was removed. The milking was continued chiefly to 

 prevent any udder troubles during the' experiment, and to main- 

 tain normal conditions. The temperature was taken but once a 

 day, at noon. 



In comparing the temperature records of these six animals, it 

 was noticed that the three animals which received the bovine cult- 

 ures had a high temperature immediately after the inoculation, 

 which lasted until the death of the bull and about three weeks for 

 the remaining two animals. At the same time, no such elevation 

 of temperature was recorded for the animals receiving the three 

 sputum cultures. There was but one well-defined rise of tempera- 

 ture in case of the yearling, from the thirteenth to the sixteenth 

 day after inoculation. The other irregularities are probably due 

 to the fact that the temperature was usually taken after these 

 animals had been in the enclosure in the sunshine for several 

 hours. Those with the high temperature were kept much of the 

 time in the cool barn in the morning, which probably depressed 

 the fever curve somewhat. These temperature records are given 

 at the end of this article. After the period of fever no other 

 elevations were noted up to the close of the experiment. 



The young bull, inoculated with bovine culture III., showed, be- 

 sides the prompt onset of a high temperature, general and local 

 disturbances about a week after the inoculation. The breathing 

 became rapid, the appetite had partly gone. Emaciation and 

 weakness supervened. He was unable to get up September 11, 

 and died the following night. 



