SPECIAL STAINS 89 



man's, and Romanowsky's are members. To use this stain, a 

 blood or pus film is spread and air dried. The stain is then run 

 on the slip, or slide, for one minute. After this time slowly drop 

 distilled water in quantity similar to that of stain used. This is 

 when the true staining takes place. After three minutes wash in 

 distilled water, dry and mount. Nuclei, malarial parasites, trypano- 

 somes, and bacteria are stained blue; red cells are stained pinkish- 

 orange; while the granules of the leucocytes are stained pink, lilac, 

 or blue, depending upon their character. 



Giemsa's Stain. 



This stain is used for demonstrating the newly discovered organ- 

 ism of syphilis Treponema Pallidum (Spirochate Pallida) and is 

 prepared as follows: 



Azur II Eosin 3 grams. 



Azur II 8 grams. 



Glycerine C. P 250 c.c. 



Methyl alcohol 250 c.c. 



1. Air dry the specimen. 



2. Harden and fix in absolute alcohol. 



3. Dilute stain with distilled water, using one drop of stain to each cubic 

 centimeter of water. 



4. Cover preparation with dilute stain 15 minutes. 



5. Wash in running water. 



6. Blot and mount. 



Capsule Staining. 



Bacteria are often covered with capsules that are difficult to stain, 

 and special methods have been devised to demonstrate them. 



Welch's Method. 



i. Cover-glass preparations are made in the usual manner, and over the film 

 after fixing, glacial acetic acid is poured. 



