STAINING DIPHTHERIA BACILLI 



93 



The most important steps in flagella staining are to clean the 

 cover-slips thoroughly, to mix the culture with water and have no 

 culture media with it, to fix gently, and not to overheat the stain. 

 Even in expert practised hands it is not always easy to demonstrate 

 flagella readily. 





4 .* ' 

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FIG. 22 B. Diphtheria stained by Neisser's method. (Williams.) 



Neisser's method of staining the diphtheria bacillus. 

 Two stains are needed: (Fig. 22.) 



A. Methylene blue i gram. 



95 percent alcohol 20 c.c. 



Water 950 c.c. 



Mix and add 



Glacial acetic acid 50 c.c. 



B. Vesuvin 2 grams. 



Distilled water 1000 c.c. 



The staining steps are as follows: 



1. Prepare film, fix and dry. 



2. Pour on "A" for thirty seconds. 



