Il6 BACTERIOLOGICAL LABORATORY TECHNIC 



layer of paraffine excludes the air. Flasks filled with bouillon, or 

 tubes filled with bouillon, or melted agar may be inoculated with an 

 anaerobic culture, but the filling of the vessel with the medium must 

 be absolute so that no space is left for air, otherwise the organisms 

 may not grow. Roux employs a long sterile glass tube, which he 

 completely fills with melted agar inoculated with the organism he 

 wishes to grow. The ends of the tube are then sealed in a bunsen 

 flame and there being no air, anaerobic conditions are fulfilled, 



FIG. 36. A streak made in agar by a needle inoculated with anaerobic bacilli 

 and then covered at one spot with cover-glass. (Williams.) 



and organisms grow. After colonies appear the tube is broken at 

 a file-mark near the colony and tubes inoculated therefrom. 



Under other methods large Novy jars are used for the reception 

 of petri dishes and test-tubes. From these jars the air is withdrawn, 

 and hydrogen allowed to flow into it. A solution of pyrogallic and 

 sodium hydrate is placed in the bottom of the jar to absorb any 



