CHOLERA BACILLUS l8l 



lowed wounds, and delivery of the child in puerperal cases. 

 It produces in fatal cases the condition known as frothy organs 

 "Schaumorgane." It may be isolated from infected mat- 

 ter, feces etc., by injecting the latter into a rabbit's vein and then 

 killing the animal. The carcass is then placed in an incubator 

 and an enormous growth of the organisms follows; anaerobic condi- 

 tions favorable to growth are obtained in the blood; from the latter 

 pure cultures are easily obtained. 



Another spore forming anaerobe very close to Welch's bacillus 

 is called Bacillus enteritidis sporogenes. Its differentiation is 

 probably certain but difficult to make. 



Vincents Angina is due to an anaerobic organism of two stages, 

 as Bacillus fusiformis and Spirochata vincenti. The bacillus is a 

 fusiform irregularly staining pointed rod, 3-1 2{j. long by .3~.8// 

 wide. Under cultivation it grows out into forms such as are seen 

 with it in smears from the diseased throat, that is, long, wavy, uni- 

 formly stained, flexible, pointed ended spirals. The bacillus forms 

 endospores chiefly at the end. Obligate anaerobe, requiring 

 serum ascitic fluid or glycerin. Colonies delicate and whitish. 

 Gas in glucose media. Litmus milk only decolorized. Gives a 

 fetid odor on all cultures. No specific immunity reactions known. 



SPIRILLACE^E. 



CHOLERA BACILLUS. 



Vibrio Cholerae. Koch. 



Spirillum Cholera. . (Fig. 64.) 



Cholera Bacillus. 



Comma Bacillus. 



Morphology and Stains. Curved or bent rods, the ends not 

 lying in the same plane. This bending varies greatly. Under 

 certain conditions of growth such as the presence of alcohol, or in- 

 sufficient albumin or oxygen in culture media, long spiral chains 

 are formed. It is motile, has one terminal flagellum, and like 



