204 BACTERIA 



Vital Requirements. Grows well at room temperature, and in 

 the incubator. Nocard kept a culture at 40 C. for four months 

 and it was still virulent. 



Cultures. It thrives well on all culture media. On bouillon the 

 growth is colorless, and in masses that float and then sink; or in a 

 fenestrated pellicle on the top. On Agar. It appears in discrete 

 little roundish yellowish-white masses that resemble lichens. On 

 blood serum its growth is like that on agar, only less luxuriant. 

 On potato it is scaly, wrinkled, yellowish and dry. In milk the 

 organism flourishes, without curdling the milk or altering its 

 reaction. 



Pathogenesis. This organism is pathogenic for all the laboratory 

 animals. Sheep, dogs, wild rabbits, horses, asses, and men are 

 immune. It produces an abscess, in those animals for which it is 

 pathogenic, that discharges, with subsequent induration, ulceration, 

 and sloughing. The disease in cattle resembles glanders. 



If injected into the blood, miliary tubercles are found that 

 resemble tuberculosis. 



ACTINOMYCES MADURA. 



Actinomyces Madura. 



Streptothrix Madura, Vincent. 



Morphology and Stains. A non-motile, non- flagellated organ- 

 ism said to have spores. Its growth resembles that of Actinomyces 

 bovis. It consists of long threads that are clubbed. These stain 

 by all the basic aniline dyes and by Gram's method. 



Vital Requirements. It is a facultative aerobe. The thermal 

 death-point for the spores is 85 C. for three minutes, and 75 C. 

 for five minutes. Vegetative thread forms die at 60 C. Grows 

 best at 37 C., and scantily at room temperature. 



Cultures. Generates upon all culture media. In Bouillon. It 

 appears in little clumps which cling to the glass, and are bright red 

 in color, eventually they sink to the bottom in pale masses. In 

 Gelatine. It grows sparingly in clumps, slowly liquefying the 



