No. 4.] CATTLE COMMISSIONERS' REPORT. 569 



Time : 8.30 p.m. Xormal temperature, 



7.30 a.m. After injection, . 



9.30 a.m. After injection, . 



11.30 a.m. After injection, . 



1.30 pm. After injection, . 



3.30 P.M. After injection, . 



Degrees F. 



101. 1 



102.2 

 105.0 

 104.1 

 102.2 

 101.3 



Six weeks after inoculation this animal was killed, aud the 

 autopsy was as follows : — 



Autopsy. — Beneath the point of inoculation, upon the surface 

 of the rumen, was a firm nodule about the size of a pea, and in 

 the immediate neighborhood another similar but smaller nodule. 

 On the parietal surface of a portion of intestine a similar nodule, 

 about the size of a pea. 



In the mesentery was a nodule about the size of a large brown 

 bean. This was irregular or rough in outline, and showed numer- 

 ous minute more or less elevated tubercles, in which calcification 

 was just beginning. This presented typical microscopic appear- 

 ances of a tuberculous lesion. 



On the parietal surface of the peritoneum there were perhaps 

 twenty minute nodules, grayish-white in color, rounded in form, 

 firm to the touch, and varying in size from almost a pin point to 

 a fairly good-sized pin's head. 



On the peritoneal surface of the spleen there were four or five 

 small circumscribed nodules, from the size of a pin's head to that 

 of a very small pea. 



No other leisons were found, though a careful search was made, 

 cutting ever}' organ into small pieces. 



3Iicroscopic Examination of Above Lesions. — Sections of the 

 nodules on the surface of the intestine and the nodule in the 

 mesentery showed the typical microscopic tubercle, with necrosis, 

 giant cells and bacilli. Although many sections of the other 

 lesions (nodule on rumen, nodules on the spleen and those on the 

 parietal surface of the peritoneum) were carefully studied, I was 

 unable to obtain absolute proof of their tuberculous nature. 



Calf II. (about three weeks old ; tested with tuberculin one 

 week previous to inoculation ; mother not tested) . — The inocula- 

 tion was made exactly as in the case of Calf I. Five weeks after 

 inoculation the animal reacted to tuberculin as follows : — 



Time: 



