570 BOARD OF AGRICULTURE. [Pub. Doc. 



Five months after inoculation this calf was killed, and the re- 

 sult of the autopsy was as follows : — 



An inguinal lymphatic gland, on the side corresponding to that 

 upon which the inoculation was made, was enlarged and contained 

 several well-marked caseous tubercles. 



The omentum was studded with nodules of various sizes (from 

 scarcely visible dots to the size of a pin head), many of which 

 were isolated, again occurring as confluent masses. Some of 

 these nodules, when confluent, were the size of a brown bean, and 

 slightly calcified. Similar minute nodules were scattered here and 

 there through the mesentery, only when confluent reaching the 

 size of a pea. 



Several mesenteric glands were enlarged, and contained small 

 caseous tubercles ; other organs were normal. 



Microscopic examination of the nodules in the omentum proved 

 them to be tubercles. 



Calf III. (three weeks old ; tested one week previous to inocu- 

 lation ; from tested mother) . — The inoculation was made by in- 

 jecting one cubic centimeter of the suspension of tubercle bacilli 

 above described into the trachea. Five weeks later this calf was 

 injected with tuberculin, and reacted as follows : — 



Time: 8.30 p.m. Normal temperature, 



7.30 a.m. After injection, . 



9.30 a.m. After injection, . 



11 .30 am. After injection, . 



1.30 p.m. After injection, . 



3.30 p.m. After injection, . 



Degrees F 



101.0 

 105.3 



105.2 

 106.2 

 104.2 

 105.0 



Six weeks after inoculation the animal was killed, and the au- 

 topsy was as follows : — 



Subcutaueously at the point of inoculation was a tuberculous 

 lesion about the size of a nut, which showed several small centres 

 of caseation. 



In the neighboring superficial muscles there were several small 

 nodules, showing beginning caseation, and evidently tuberculous 

 in nature. 



Beneath the point of inoculation, in the deeper cervical muscles 

 to the right of the trachea, there was an enlargement the size of 

 a large hen's egg, reaching to the cervical vertebrte. It was fluc- 

 tuating to the touch, and contained a clear, watery fluid, in which 

 were numerous flat, yellowish-white flakes (later microscopic ex- 

 amination of these flakes showed numerous tubercle bacilli). 

 The wall of this cyst was thick and fibrous, its internal surface 



