572 BOARD OF AGRICULTURE. [Pub. Doc. 



CoNTKOL Experiments. — As control experiments of the above 

 four, two guinea pigs were inoculated, each with one-half cubic 

 centimeters of the same suspension of human tubercle bacilli that 

 was used to inoculate calves 1 to 4. 



Guinea Pig 1 was inoculated by injecting the fluid into the 

 abdominal cavity. At the end of four weeks this pig was killed, 

 and the autopsy showed : — 



Inguinal glands swollen, but not caseous. 



Both testicles markedly tuberculous and caseous. 



Tuberculosis of the penis with caseation (tubercle bacilli were 

 plenty in the caseous material of the testicles and penis). 



Guinea Pig 2. — One-half cubic centimeter of the above suspen- 

 sion of tubercle bacilli was injected into the trachea. Animal 

 killed at the end of three mouths. 



Autopsy. — Tracheal and post-pharyngeal lymphatic glands en- 

 larged, and in one instance caseous (from this, tubercle bacilli 

 were demonstrated in cover-glass preparations). 



The lungs, liver and spleen were studded with miliary tubercles. 



Exp)eriment II. — Inoculation of calves tvith human tuberculous 

 sputum. 



The sputum used was rich in tubercle bacilli, and but few other 

 organisms were present. The sputum was mixed with boiled, dis- 

 tilled water, and inoculated by means of a hypodermic syringe. 

 A small drop of this mixture showed numerous tubercle bacilli 

 when examined with the microscope. 



Calf V. (three weeks old ; from tested mother; calf not tested). 

 — Inoculation was made subcutaneously, with two cubic centime- 

 ters of the above mixture of sputum and water. 



Time: 8.30 p.m. Xormal temperature, 



7.30 a.m. After injection, . 



9.30 a.m. After injection, . 



11.30 am. After injection, . 



1.30 p.m. After injection, . 



3.30 p.m. After injection, . 



Degrees F. 



102.4 

 103.3 

 105.3 

 105.2 

 104.2 

 104.1 



This animal was killed at the end of five and one-half months. 

 The autopsy was made by one of the Cattle Commissioners, and 

 he was unable to find any pathological changes, save a slight 

 enlargement of a mediastinal lymphatic gland and a small spot in 

 the liver, which he did not consider a tubercle. A guinea pig was 

 inoculated with pieces of this gland and the lesion in the liver, and 

 at the end of two and a half months this guinea pig was killed, 

 and I was unable to find anything abnormal at the autopsy. 



