No. 4.] CATTLE COMMISSIONERS' REPORT. 573 



Calf VI. (three weeks old ; not tested ; from tested mother). 

 — The inoculation was made by injecting two cubic centimeters of 

 the mixture of sputum and water in the trachea. 



Degrees F. 



Time: 8.30 p.m. Normal temperature, . . 101.4 



7.30 AM, After iujection, .... 104.2 



9.30 am. After injection, . . . .106.0 



11.30 a.m. After injection, . . . . 106.2 



1.30 p.m. After injection, .... 105.0 



3.30 p.m. After iujection, .... 105.0 



The animal was killed four mouths and nine days after inocu- 

 lation. 



Autopsy at the Point of Inoculation. — Just beneath the skin a 

 firm, partly calcified and partly caseous tubercular mass, the size 

 of a horse-chestuut. Beneath this in the muscles were numerous 

 small, firm, partly caseous and calcified tubercles, extending for 

 several inches in the long direction of the muscles. No other 

 lesions were fouud. 



3Iicroscopic Examination. — Cover-glass preparations from the 

 caseous material of the lesion at the point of inoculation and the 

 tubercles in the muscles showed numerous tubercle bacilli. 



Calf VII. (two and a half weeks old ; not tested ; from tested 

 mother). — Inoculation the same as the preceding. 



Degrees F. 



Time: 8.30 p.m. Normal temperature, . . 102. 1 



7.30 a.m. After injection, .... 102.2 



9.30 a.m. After injection, . . . .103.0 



11.30 am. After injection, . . . . In3.1 



1.30 p.m. After injection, . . . . 102.0 



3.30 p.m. After injection, . . . .101.2 



Killed four months and nine days after inoculation. 



Autopsy. — Subcutaneously at the point of inoculation a typical 

 microscopic tubercle the size of a pea. The underlying muscles 

 contained a few small tubercles, mostly more or less calcified. 

 These lesions were similar, but by no means so extensive as in 

 the preceding case. 



Liver. — Just beneath the capsule and close to the gall-bladder 

 was a yellowish nodule the size of a very small pea, with an ap- 

 parently caseous centre. A few other smaller yellowish spots 

 were also found, but all were just beneath the capsule of the 

 liver. No other lesions were found. 



Microscopical examination of two of the nodides in the liver 

 showed them to be tubercles in histological structure, though no 



