574 BOARD OF AGRICULTURE. [Pub. Doc. 



bacilli could be found. Sections were also made through other 

 similar-looking lesions, but they proved to be non-tubercular in 

 structure. 



Control Experiments. — As control experiments of the pre- 

 ceding three, two guinea pigs were inoculated, each with one-half 

 cubic centimeter of the same sputum mixture which had been 

 used for the calves. 



Guinea Pig 3 was inoculated subcutaneously. This animal 

 died five days after inoculation. 



Autopsy. — Extensive fibriuo-purulent inflammation, extending 

 over nearly the whole surface of the abdomen, both subcutane- 

 ously and between the abdominal muscles. Microscopic exam- 

 ination of the purulent material showed great numbers of pneumo- 

 cocci, and pure cultures of the same organisms were obtained from 

 the heart's blood. (Cause of death, infection with pneumococci.) 



Guinea Pig 4. — Inoculation made into the trachea. 



Killed at the end of five and a half weeks. 



Autopsy. — At the point of inoculation a tubercular abscess 

 the size of a horse-chestnut. The pus in this abscess contained 

 many tubercle bacilli. 



Lungs, Liver and Spleen : milary tuberculosis. 



Summary. 



From so few experiments it is impossible to claim any absolute 

 scientific evidence that cattle are less susceptible to the human 

 tubercle bacillus than they are to the bovine. For such a state- 

 ment to be of scientific importance, many experiments in this line 

 must be made, and, at the same time, calves must be inoculated 

 with the bovine tubercle bacillus for comparison. 



Reviewing the results of these few experiments upon calves, we 

 have the following : — 



1. Of the four calves inoculated with a pure culture of the 

 human tubercle bacillus, only three showed any post-mortem 

 lesions of tuberculosis, and in no instance were these changes 

 extensive. The most marked lesions were in the immediate vicin- 

 ity of the point of inoculation (in abdominal inoculation, in the 

 omentum and peritoneum ; intratrachial inoculation, in the muscles 

 of the neck and the lymphatic glands of the same region. It is 

 possible that the needle did not enter the trachea in some of these 

 cases.) The lesions elsewhere in the body were very minute, and 

 only found by careful search. There was never anything approach- 

 ing a general infection, as was the case in one of the control ani- 

 mals (Guinea Pig No. 2). That the lesions in Guinea Pig 1 

 remain localized in the penis and testicles is inexplicable. 



