529 



usual meat-infusion media. H, is practically indifferent to moderate 

 variations in reaction, growing equally well upon neutral, slightly acid, 

 or slightly alkaline culture media., (llycerin or small quantities of 

 glucose added to media seem to render them more favorable for the 

 cultivation of this bacillus. 



Upon agar the colonies show little that is characteristic. They 

 appear after twenty-four hours at 37.5 C. as yellowish-white spots, 

 at first transparent, later more opaque. They are round, with an even 



, ^MV 



* >;, 



fr"<K * .'' -. * V 



^- % 



*t. 



FIG. 110. GLANDERS BACILLUS. From potato culture. (After Zettnow.) 



border, and microscopically appear finely granular. The older the cul- 

 tures are, the more yellow do they appear. 



On gelatin at room temperature, growth is slow, grayish- white, and 

 no liquefaction of the gelatin occurs. Growth upon this medium is 

 never abundant. 



In broth, there is, at first, diffuse clouding, later a heavy, tough, 

 slimy sediment is formed. At the same time the surface is covered with 

 a similarly slimy pellicle. The broth gradually assumes a dark brown 

 color. 



In milk, coagulation takes place slowly. In litmus milk, acidifica- 

 tion is indicated. 



The growth upon potato presents certain features which are diagnos- 

 tically valuable. On potatoes which are not too acid growth is abundant 

 and within forty-eight hours covers the surface as a yellowish, trans- 



