732 



Culture media, for colon-typhoid dif- 

 ferentiation, 132 

 glassware preparation in, 113 

 glycerin for, 126 

 ingredients of, 115 



choice of, 116 



lactose litmus-agar, 129, 134 

 litmus milk, 130 

 milk, 130 

 potato for, 130 

 glycerin, 130 

 preparation of, 113 



general technique of, 113 

 process of, 124-40 

 reaction of, 119 

 serum, 131 



Hiss' serum water, for fermenta- 

 tion tests, 132 

 Loeffler's, 131 

 slanting of, 123 



special, 132 

 sterilization of, 121 

 filtration in, 122 

 heat in, 121 

 titration of, 117 



color indicator in, 117 

 process of, 117 



for alkaline media, 118 

 reaction of, 117 



adjustment of, 119 

 tubing of, 121 



Cultures, attenuated, in active immu- 

 nization, 193 

 Cultures, incubation of. See Incubation 



of cultures 

 Cytase, 279 



Cytoryctes variola?, 658 

 Cytotoxins, 201 



specific, injury to organs by, 201 



DARK-FIELD condenser. 597 



Decay, action of, 44 



Defensive factors of animal organism, 



189 



Degenerative forms of bacteria, 20 

 Denitrifying bacteria, 52 



activities of, 53 



occurrence of, 53 



Destruction of bacteria, 62 

 by chemical agents, 73 

 See also Disinfectants 

 gaseous, 88 

 in solution, 73 

 inorganic, 73 

 organic, 77 



by physical agents, 62 

 drying, 62 

 electricity, 65 

 heat, 65 

 light, 63 

 Diarrheal diseases traced to milk, 



705 



Diastase. See Amyjase 

 Differential stains in staining of bac- 

 teria, 102 



Diphtheria, tracing of, to milk, 705 

 Diphtheria antitoxin, 216 A 

 anaphylaxis in injections of, 296 

 normal, 205 

 production of, 216 

 horses used in, 216-17 

 technique of, 217 

 toxin for, 216 

 stable, 206 

 standardization of, 218 



concentration and purifying in, 



219 



technique of, 218 

 unit for, 205 



Diphtheria bacillus, 512"^ 

 bacilli similar to, 523 

 Bacillus Hoffmanni, 523 

 cultivation of, 523 

 morphology of, 523 

 staining of, 523 

 Bacillus xerosis, 525 

 cultivation of, 526 



differentiation of, from other ba- 

 cilli, 526 



other bacilli, 526 

 biological characteristics of, 515 

 cultivation of, 516 

 degenerative forms of, 19 

 differentiation of, from other forms, 

 107, 523 et seq. 



