32 



2 ee. of chloroform. After the separation of the liquids^ 

 2 cc. of the chloroform layer are tested Math 4 cc. of a 

 solution of 1 gram of crystallized acetate of zinc in a liter 

 of 95% alcohol (shelf reagent). At the junction of the 

 two layers the green fluorescent ring characteristic of uro- 

 bilin will appear, and on shaking, a fluorescence which is 

 rose-colored by reflected light will be distinguished 

 throughout the liquid. 



Another test is to add ammonia to the urine until 

 distinctly alkaline, filter, and to the filtrate add a little 

 10% chloride of zinc solution. A green fluorescence should 

 appear, and if examined with the spectroscope, a Charac- 

 teristic band should occur. (See Fig. 16.) 



Leucin and Tyrosin are pathologic constituents of urine. 

 They are normal products of pancreatic digestion and under 

 ordinary conditions are carried, after absorption, to the liver, 

 where they disappear, presently undergoing decomposition. 

 When present in the urine these bodies ^ire usually considered 

 pathognomonic of acute yellow atrophy of the liver, although 

 they are likewise stated to be present in the urine in certain 

 rare cases of acute phosphorus poisoning associated with hepatic 

 atrophy, due to typhoid fever, etc. 



Phenol. According to Tereg and Munk the horse excretes 

 in the urine about 10 grams of tribromphenol in 24 hours. The 

 tribromphenol is equivalent to 3 grams of phenol daily. Great 

 importance is laid by these observers on the excretion of phenol, 

 a process which is suspended during intestinal complaints, par- 

 ticularly colic, and is, according to them and others, a cause of 

 the rapid death in these affections, produced by the toxic effect 

 of the unexcreted phenol. The production of phenol in the 

 healthy body is greatly influenced by diet, being largest on rye 

 and hay, one part. peas and two parts oats, and on hay alone; it 

 is smallest on rye alone, and next smallest on oats and hay. Sal- 

 kowski is inclined to regard the excretion of 3 grams of phenol 

 daily as too high. 



