50 



urine. The amount of uric acid for the 24 hours can be calcu- 

 lated by the following formula, supposing that 1250 cc. was the- 

 amount of the urine passed. 



0.001175X5 



10 



•X 1250=0.7343 gm. 



Estimation of Uric Acid by Weight. 20 cc. of hydrochloric 

 acid are added to 200 cc. of urine and the mixture set aside for 24 

 hours. Uric acid crystals form and collect on the sides of the 

 vessel. This may be collected on a weighed filter and washed 

 thoroughly with water. The filter and uric acid are dried and 

 weighed until there is no further loss of weight. The weight of 

 the filter paper is deducted and the result gives the amount of 

 uric acid in 200 cc. of urine, from which the amount in 24 hours 

 can be calculated. 



Urea. Doremus Ureometer Test. Fill the long arm an:l 

 bend of the ureometer with the hypobromite solution. (Hypo- 

 bromite of sodium is prepared by mixing 2 cc. of bromine with 



Fig. 13. Hind's modification of 

 Doremus Ureometer. 



23 cc. of a solution of caustic soda, 40 grams to 100 cc. of distilled 

 water. To this mixture add an equal volume of distilled water) . 



