52 



Caution. The coagulable substance- found in the 

 urine of Bright 's disease is a mixture of ' ' serum-albumin ' ' 

 and ' ' serum-globulin " or " para-globulin. ' ' Saturation of 

 the urine with crystallized magnesium sulphate precipi- 

 tates the serum-globulin, leaving the serum-albumin in 

 solution. 



Centrifuge Test. Fill the graduated tube with urine 

 up to the 10 cc. mark; add 5 cc. of Esbach's reagent. In- 

 vert the tube several times in order to thoroughly mix the 

 fluids. Place the tube in the centrifuge and revolve for 

 three minutes. Read off the amount of the precipitate; 

 each 0.1 cc. is the equivalent of 0.21 of a gram of dry al- 

 bumin per 1000 cc. of urine. 



Ferrocyanide Test with Centrifuge. The percentage 

 tube is filled to the 10 cc. mark with the urine to be tested. 

 Add 1 cc. of acetic acid and 4 cc. of a 5% solution of 

 potassium ferrocyanide. Proceed as in the preceding test. 

 Each 0.1 cc. of precipitate is the equivalent of 0.21 gram 

 of dry albumin per 1000 cc. of urine. 



Sugar. Einhorn's Fermentation Saccharometer. 

 Determine the specific gravity. Add 10 cc. of the sus- 

 pected urine to 90 cc. of distilled water. Put in a flask, 

 add about one gram of compressed yeast and agitate 

 choroughly. Pour 10 cc. of the mixture into the 

 bulb of the saccharometer and, by inclining the 

 apparatus, the fluid will displace the air in the 

 cylinder and remain there by atmospheric pres- 

 sure. Be sure that no air bubbles remain in the 

 cylinder. It is always well to test a normal urine 

 at the same time and in the same way as a con- 

 trol. The mixture of normal urine with yeast 

 will, on the following day, have only a small 

 bubble on the top of the cylinder. If, in the sus- 

 pected urine, there is also present at the top of 

 the cylinder a small bubble, no sugar is present; 

 but if there is a much larger volume of gas (CO2) 

 it is certain that the urine contains sugar. The appar- 

 atus should remain in a moderately warm place. As a 

 result of fermentation the sugar is broken up into alcohol 



