53 



and CO2. The changed level of the fluid in the cylinder 

 shows that the reaction has taken place and indicated by 

 the^ numbers the approximate quantity of sugar present. 

 The -scale on the tube is empirical and indicates directly 

 the percentage in the urine. 



Shieb's Test for Sugar in the Urine. 

 Solution No. 1. 



Ammonium Sulphate (purest) 1.2 grams. r--- 



Copper Sulphate (purest) - 2.6 grams. 



< Distilled Wate]^ « - - - 50. cc. 



Solution No. 2. 



Caustic Potash C. P. - - 20 grams. 



Distilled Abater - - - - 50 cc. 



Dissolve and when cool, add 



Glycerine • - - - - . 50 cc. 



Ammonia water 0.9G0 sp. gr. 300 cc. 



Add No. 1 to No. 2 and dilute the w^ole to 500 cc. 

 with distilled water. Stopper securely and shake till 

 thoroughly mixed. 



Heat one dram of this solution in a test tube to boil- 

 ing. Add the urine drop by drop, at slow intervals, boil- 

 ing after each addition until the blue color has been dis- 

 charged and the fluid has a light amber color or is colorless. 



If the solution is decolorized by 3 minims of urine it 

 contains 9 to 10 grains of sugar per oz. 



If the solution is decolorized by 4 minims of urine it 

 contains 7 to 8 grains of sugar per oz. 



If the solution is decolorized by 5 minims of urine it 

 contains 5 to 6 grains of sugar per oz. 



If the solution is decolorized by 6 minims of urine it 

 contains 4 grains of sugar per oz. 



If the solution is decolorized by 7 minims of urine it 

 contains 3 grains of sugar per oz. 



If the solution is decolorized by 9 minims of urine it 

 contains 2 grains of sugar per oz. 



If the solution is decolorized by 10 to 17 minims of 

 urine it contains 1 grain of sugar per oz. 



If the urine contains more than 10 grains of sugar to 



