ISOLATION AND ESTIMATION OF TRYPTOPHAN 31 



Caseoglutin 



Conglutin (yellow lupin) 



Edestin (oil seeds) 



Globulin (squash seed) 



Glutencasein (wheat gluten 



Legumin (pea) . 



Plant-fibrin 



Plant-gelatin 



Tyroalbumin 



Vitellin (squash seed) 



o'o8 per cent. 



O'o6 



0-58 



o 61 



0-07 



0-83 



o'O4 



0-42 



0*05 



0*30 



1 69-9 per cent, dry substance. 



These values are not in agreement with those found by Fasal in 

 the cases of milk albumin, caseinogen, edestin and keratin (horn). 



In all cases the time of digestion of the protein was presumably 

 twenty-four hours, and in this time the whole of the tryptophan is not 

 likely to be liberated from the protein. A maximal colour reaction 

 for tryptophan with bromine water as stated by Hopkins and Cole is 

 only obtained after several days, and the rate of digestion of proteins 

 is not likely to be the same ; keratin is not dissolved by trypsin and 

 hence the low value.* 



The tryptophan content of the kidneys in normal and pathological 

 conditions has been determined by this method by Kurchin [1914]; 

 normal kidneys contained slightly more than pathological. 



