CONTENTS OF PART I. 



PAGE 



INTRODUCTION i 



I. HYDROLYSIS - 9 



II. THE ISOLATION AND ESTIMATION OF THE UNITS - 15 



A. THE MONO-AMINO ACIDS 1 7 



TYROSINE - 1 7 



(1) Isolation and Gravimetric Estimation 17 



(2) Colorimetric Estimation - - 20 



(3) Estimation by Bromination - 22 

 CYSTINE - 24 



(1) Isolation and Gravimetric Estimation - 24 



(2) Separation of Cystine and Tyrosine - 25 

 TRYPTOPHAN - 27 



(1) Isolation and Gravimetric Estimation - 27 



(2) Colorimetric Estimations - - 28 



(3) Estimation by Bromination - - 32 



THE OTHER MONO-AMINO ACIDS - 33 



(1) Isolation of Glutamic Acid as Hydrochloride - 33 

 (id) Isolation of Glutamic Acid as Zinc Salt 34 

 (ib) Isolation of Glutamic and Aspartic Acids as Silver Salts 34 

 (it) Isolation of Glutamic and Aspartic Acids as Calcium Salts 34 



(2) Esterification - 35 



(3) Isolation of Glycine as Ester Hydrochloride - - 36 



(4) Extraction of the Esters of the Amino Acids - - 37 



(5) Fractional Distillation of the Esters in Vacua - 40 



(6) The Isolation of the Individual Mono-amino Acids - 42 



(a) Proline 43 



(b) Glycine, Alanine, Valine, Leucine, and Isoleucine 44 

 i. Separation of Valine from Leucine and Isoleucine - - 45 

 ii. Valine - 46 

 iii. Leucine and Isoleucine - 47 

 iv. Separation of Valine and Alanine - 48 

 v. Separation of Glycine and Alanine - -51 



(c) Phenylalanine - 52 



