Quantitative Bacteriological Examination. 35 



EFFECT OF SIZE OF VESSEL UPON THE MULTIPLICA- 

 TION OF WATER BACTERIA DURING STORAGE. 



(WHIPPLE, 1901.) 



1 Average of five plates. 



Wolffhiigel and Riedel (Wolffhiigel and Riedel, 1886) 

 noted the dependence of this multiplication on the air- 

 supply, vessels closed with rubber stoppers showing 

 lower numbers than those plugged with cotton. Simi- 

 larly, Whipple found that the multiplication of bacteria 

 was much greater when bottles were only half full than 

 when they were filled completely; and also, as shown 

 in the above table that the size of the bottle markedly 

 influenced the growth. 



An important series of investigations by Kohn (1906) 

 suggests that this phenomenon of multiplication during 

 storage may be due in part to -the solution of certain 

 constituents of glass which favor bacterial life, since the 



