PARASITISM IN FUNGI 141 



is usually effected during the first twenty-four hours, and 

 the tip of the germ-tube can be seen preparing to enter 

 the stomata in abundance during the second day. I have, 

 moreover, succeeded in fixing, hardening, and staining the 

 germ-tubes and their nuclei, the preparations being then 

 mounted in balsam. 



'The young germ-tube grows very rapidly, and the 

 nucleus of the spore soon passes into it, and by suitable 

 methods of hardening and staining can be detected either 

 somewhere along its course or near the tip as it approaches 

 a stoma to enter it. In some cases two or more nuclei are 

 seen in the tube, and the resemblance to a germinating 

 pollen-grain is striking. The first stage in inoculation is 

 evident in the swelling of the tip of the germ-tube over 

 the stoma into a thin vesicle, into which all the nucleated 

 protoplasm derived from the spore passes. This vesicular 

 swelling the so-called appressorium then puts a thin 

 process down through the stomatal slit, which process 

 again swells into a vesicle on the inner face of the stoma 

 and projecting into the respiratory cavity, and the proto- 

 plasmic contents are passed through from the external 

 appressorium to the internal vesicle. 



' The latter quickly bulges out at one or more points in 

 the form of a tube the true infecting hypha into which 

 the whole, or part, of the vesicular protoplasm passes and 

 its nucleus soon divides therein.' 



Brefeld, Sckles. Ges. Vaterl. Cultur., Zool.-Bot. Sect., 

 p. 17 (1900). 



Gibson, New Phytologist, 3, p. 184 (1904). 



Massee, Phil. Trans. Roy. Soc., ser. B., 197, p. 7 (1904). 



Miyoshi, Bot. Ztg., p. i (1894). 



