504 HISTOLOGY 



be ripened by the addition of 10 c.c. of j per cent. aq. sol. of potassium 

 permanganate. 



Tissues must be fixed in Zenker's fluid. Sections are transferred 

 from water to \ per cent, aqueous solution of potassium permanganate 

 for 3 to 5 minutes, washed in water, and put for 5 to 10 minutes in 5 per 

 cent, aqueous solution of oxalic acid. Wash thoroughly in several changes 

 of water, and stain in the haematoxylin solution for 12 to 24 hours. Trans- 

 fer directly to 95 per cent, alcohol for not more than i or 2 minutes, fol- 

 lowed by absolute for paraffin sections. Clear in xylol, using the filter- 

 paper blotting method for celloidin sections (see p. 508). 



Neuroglia, myoglia, and fibroglia fibrils and fibrin are stained blue; 

 collagen fibrils reddish-brown; mitotic figures well shown. 



Mallory's Connective Tissue Stain. 



Anilin blue soluble in water (Griibler) 0.5 gm. 



Orange G (Griibler) ' 2.0 gm. 



Phosphomolybdic acid, i per cent. aq. solution 100. o c.c. 



Paraffin or celloidin sections of material fixed in Zenker's fluid are 

 transferred from water to a 0.2 per cent, aqueous solution of acid fuchsin 

 for 5 to 20 minutes. Transfer directly to the anilin blue solution and stain 

 for 20 minutes or longer. Wash in several changes of 95 per cent, alcohol. 

 Clear celloidin sections in oil of origanum. Paraffin sections are passed 

 through absolute, absolute and xylol, to xylol. 



Fibrils of connective and reticular tissue, amyloid, and mucus stain 

 blue; nuclei, cytoplasm, muscle, axis cylinders, and neuroglia fibers stain 

 red; red corpuscles and myelin, yellow. 



Weigert's Resorcin-fuchsin. Boil, in an evaporating dish, 2 gm. 

 of fuchsin and 4 gm. of resorcin in 200 c.c. of water. When it is boiling 

 briskly, add 25 c.c. of liquor ferri sesquichlorati. Stir and boil for 5 

 minutes. Cool and filter. Allow the precipitate to dry; return the filter 

 paper with precipitate to the dry dish; add 200 c.c. of 95 per cent, 

 alcohol and boil, stirring constantly. Fish out the paper. Cool and 

 filter; add alcohol until the volume of 200 c.c. is reached and add 4 c.c. 

 of hydrochloric acid. 



From 95 per cent, alcohol the sections, preferably fixed in alcohol or 

 formaldehyde, are transferred to the stain for 20 minutes to an hour. 

 Wash in 95 per cent. Clear in xylol by the blotting method (p. 508). 



The elastic fibers are stained a deep purple. The remainder of the 

 tissue should be nearly or quite colorless. If other parts are affected, the 

 sections should be washed in alcohol containing 0.5 per cent, of hydro- 

 chloric acid. A light nuclear stain with alum hasmatoxylin after the elas- 

 tic tissue has been stained will increase the value of the specimen. 



Scharlach R. Frozen sections of fresh or formalin fixed material 

 are stained from 15 minutes to over night in a saturated solution of the 





