POISONING BY PLANTS. GENERAL PRINCIPLES 27 



8. See KOLMER, JOHN A. : A Practical Text-book of Infection, Immunity and Specific 

 Therapy. Philadelphia, W. B. Saunders Company (second edition), 1917, page 898, 

 for the following experiment with phy to toxins: Prepare a i per cent, suspension of 

 washed rabbit and guinea-pig corpuscles. Into a series of six small test-tubes place 

 increasing doses of ricin or abrin solution as follows: o.i, 0.2, 0.3, 0.4, 0.5, and 0.8 c.c. 

 Add i c.c. of rabbit-cell emulsion to each and sufficient normal salt solution to make 

 the total volume in each tube equal to 2 c.c. A seventh tube is the corpuscle control 

 and contains i c.c. of the .erythrocyte suspension and i c.c. of salt solution. Prepare 

 a similar series of tubes with the guinea-pig erythrocyte suspension. Shake the tubes 

 gently and incubate for two hours. 



Queries. Do any of the tubes show hemolysis or hemagglutination? Is the action 

 the same with both bloods? Does the plant toxin show a selective affinity? 



