82 THE CHEMISTRY OF THE TISSUES AND ORGANS. 



viscidity, and it was the absence of this character which led me to the 

 erroneous conclusion that no nucleo-proteid had gone into solution. 



The sodium sulphate extract contains two proteids, one which co- 

 agulates at 4S-50 C., the other at 75 C. The first, which I called cell 

 globulin-a, is really a globulin ; it yields no nuclein on gastric digestion ; l 

 but the second, which I called cell globulin-j6, though like a globulin in its 

 solubilities, is really the same nucleo-proteid which by treatment with 

 other salts is rendered viscid. 2 That this substance is related to, if not 

 identical with, the fibrin ferment or its zymogen (Pekelharing) has been 

 rendered probable by the researches of Pekelharing and myself. 



The albumin is only present in minute quantities ; its properties are 

 like those of serum albumin, and it may partly arise from blood or 

 lymph imperfectly washed away frin the cells. 



Proteases and peptone, when present, are the result of post-mortem 

 changes, or of manipulations during the processes employed in separating 

 the other proteids. 



Myosin is absent. 



Lilienfeld 3 has carried out a similar research on the chemistry of 

 cells which he obtained from the thymus, by the usual means of pressure 

 and the centrifuge. He found a proteid corresponding to cell globulin-a 

 coagulating at 48 C., and another corresponding to cell albumin 

 coagulating at 73-75 C. The nucleo-proteid which he obtained by my 

 sodium chloride process contained C, 53*46 ; H, 7 '64 ; N, 15 '5 7, and P, 

 0*433 per cent. The alcoholic extract of the cells contained protagon, 

 amido-valeric acid, inosite, and monopotassium phosphate. 



By Wooldridge's method he obtained the nucleo-proteid he has 

 called " nucleo-histon " (see p. 68), and he considers that this, in part at 

 any rate, is derived from the nuclei. Its percentage composition is C, 

 48-46; H, 7'0 ; N, 16-86 ; P, 3-025 ; and S, 0701. The action of artificial 

 gastric juice, or of 0'8 per cent, hydrochloric acid, on this, is to separate 

 the nuclein from the proteid, which goes into solution as peptone. The 

 nuclein contains 4*991 per cent., and the nucleic acid prepared from 

 this 9-94 per cent., of phosphorus. 



In the following table he gives the quantitative composition of 

 leucocytes : 



Water . 88-51 



Solids 11-49 



One hundred parts of the solids contain 



Total phosphorus 3 -01 



Total nitrogen 15*03 



Proteid 176 



Nuclein 6878 



Histon (i.e. proteid part of the nucleo-proteid) . 8 - 67 



Lecithin 7*51 



Fat 4*02 



Cholesterin 4*40 



Glycogen 0*80 



1 Halliburton, Journ. Physiol., Cambridge and London, 1892, vol. xiii. p. 806. 



2 Ibid., 1895, vol. xviii. p. 312. Pekelharing showed this also to be the case. 



3 Ztschr.f. physiol. %em., Strassburg, Bd, xviii. S. 473. 



