CONTENTS. 



INTRODUCTION. 



Histological requisites 



The use of the microscope 



Artificial illumination ..... 



Cleaning of the objectives . . . . 



Method of focussing an object . 



Rules for examining an object 



Preparation of a simple object . 



Methods of applying a cover-glass . 



Method of cleaning a cover-glass 



Measuring cover-glasses . . . . 



Placing a section on a slide 



Delineation of an object . . . . 



Methods of studying living protoplasm 



Methods of applying heat to miscroscopic 



objects 



Methods of applying vapours to objects 



PAGE 



xix 



xxi 



xxiii 



xxiii 



xxiii 



xxiv 



xxiv 



xxiv 



xxv 



xxv 



xxvi 



xxvi 



xxvii 



xxviii 

 xxix 



PAGE 



Preliminary preparation of the tissues . . xxx 



Methods of hardening the tissues . . xxxi 



Methods of softening the tissues . . . xxxiii 



Dissociation of the tissues .... xxxiv 



Methods of cutting sections . . . . xxxvi 



Microtomes xxxvii 



Methods of staining the tissues . . . xlii 



Aniline dyes ...... xliv 



Metallic solutions xlv 



Double- staining xlvi 



Treble-staining . ..... xlvii 



Staining reagents ..... xlvii 



Methods of mounting sections . . . xlviii 



Labelling and preserving sections . . xlix 



Methods of injecting blood-vessels . . 1 



Methods of injecting lymphatics . . Iv 



PRACTICAL WORK. 



Blood of newt or frog . 



Human blood 



Blood-crystals 



Epithelium 



Endothelium 



Cartilage 



Connective tissue 



Bone 



Muscular tissue . 



Nerve-tissue . 



Heart and blood-vessels 



Circulation of the blood . 



Larynx, Trachea, and Lungs 



Lips and Tongue . 



Tooth .... 



Salivary glands 



Tonsils .... 



QEsophagus and Stomach 



Intestine .... 



Liver 



Pancreas .... 



Lymphatic system . 



Lymphatic glands 



Spleen .... 



Thymus .... 



PAGE 

 I 



5 



6 



8 



13 



14 



20 



3 

 36 

 42 

 47 

 52 

 S3 

 60 

 62 

 64 

 65 

 65 

 67 



7 1 



75 

 76 



77 

 78 

 81 



Ductless glands .... 

 Supra-renal capsules 



Kidneys 



Ureter 



Bladder 



Skin ..... 

 Spinal cord and medulla oblongata 

 Cerebellum and cerebrum 

 Nerve-ganglia .... 

 Eye . . . . . 



Cornea 



Nose 



Semicircular canals and cochlea . 

 Testis ... 

 Epididymis 



Ovary 



Fallopian tube .... 

 Uterus ... 

 Mammary gland 



Milk 



Placenta 



Deciduse .... 



Amnion 



Umbilical cord 



PAGE 

 82 

 82 

 84 

 88 

 89 

 90 

 96 

 100 



102 

 104 

 IO4 

 III 

 H3 



116 

 118 



120 

 121 

 121 

 122 

 122 

 122 

 I2 4 

 I2 4 

 124 



INDEX 



125 



