IN TROD UCTION. xlvii 



TREBLE-STAINING. 



This method has recently been practised by Dr. Heneage Gibbes. The process is tedious, 

 and not so satisfactory as double-staining. A combination of picrocarmine and iodine-green 

 for sections of the small intestine, including a Peyer's patch, gives an excellent and most 

 instructive effect (p. 69), in fact a perfect treble stain. 



Other combinations, as picrocarmine, eosin, and iodine-green, have been tried. 



GOLD CHLORIDE AND AN ANILINE DYE. 



This process is alluded to in connection with the study of the parts in the tail of a rat 

 (p. 25). The tissue is first treated with gold chloride and then decalcified, and the sections 

 are stained with rosein, and then with iodine-green, or with iodine-green alone. The effects 

 produced on the developing bone especially are most remarkable and striking. This method is 

 capable of further extension. 



LIST OF STAINING REAGENTS. 



Ordinary staining reagents. 



1. Logwood or Hzematoxylin. 3. Picrocarmine. 



2. Carmine. 4. Eosin. 



A niline dyes soluble in ivater. 



1. Magenta, or Rosanilin. 3. Aniline blue-black. 



2. Aniline blue. 4. Methyl-aniline. 



5. Iodine Green. 



Aniline dye soluble in spirit. 

 Rosein. 



Metallic Substances. 

 I. Silver Nitrate. 2. Gold chloride. 3. Osmic Acid. 



Many other dyes, as iodine, indigo-carmine, &c., are recommended, but the above arc what 

 the author has found to be really useful. 



