INTRODUCTION. li 



INJECTION FLUIDS. 



(A.) INJECTION-MASSES FLUID AT ORDINARY TEMPERATURES. 



1. Watery Solution of Brucke's soluble Berlin-blue. Dissolve 10 grms. of Briicke's soluble 

 blue in 500 c.c. of distilled water. 



Brucke's soluble blue can be purchased in the market ; but if it be desired to make it, the 

 following is the formula. The process is rather tedious, (a) Dissolve 217 grms. of potassic 

 ferrocyanide in I litre of distilled water. (f>) Make one litre of a 10 per cent, solution of ferric 

 chloride. Add to each of these solutions (a and b~) two litres of a saturated solution of sodic 

 sulphate. Then add the ferric chloride to the ferrocyanide, and stir the fluids whilst they 

 mix. The precipitate is filtered through a conical bag of new flannel, and washed with a 

 small quantity of water. The first washings are returned. The washing is repeated for 

 several days, until the solution when passed through filter paper is of a deep blue colour. It 

 is then soluble. The precipitate is collected from the flannel, pressed between folds of 

 blotting paper, dried, and broken into small pieces. After the blood-vessels of an organ have 

 been filled with this solution it is placed in equal parts of methylated spirit and water, to 

 which a trace of hydrochloric acid is added, as this prevents the diffusion of the blue ; or the 

 organ may be hardened in Muller's fluid or picric acid. In some cases it is advisable to heat 

 the injection-fluid to a temperature of 40 C. before injecting it into the vessels. A little 

 glycerine .added to the mass makes it flow more easily. 



2. Richardson's Blue. (a) Dissolve 10 grains of ferric sulphate in 10 oz. of water, (b) 

 Dissolve 32 grains of potassic ferricyanide in I oz. of water. Mix a and b, and then add 

 water to 20 oz. 



(B.) INJECTION-MASSES NOT FLUID AT ORDINARY TEMPERATURES. 



1. Soluble Blue and Gelatine. Soak 50 grms. of the best gelatine (Cox or Coignet) in 

 water for several hours. Pour off the water which is not absorbed by the strongly swollen-up 

 gelatine, and melt the gelatine over a water-bath. The gelatine may be placed in an ordinary- 

 jelly can or tin vessel, and this placed in a pan of water on the fire, if a water-bath is not 

 at hand. A two per cent, watery solution of Berlin-blue is prepared (p. li.), and heated to 

 the same temperature as the gelatine. Take 250 c.c. of this fluid, and gradually add to it the 

 solution of gelatine, the mixture being stirred all the time. Whilst still hot the mixture is 

 filtered through flannel, and then kept at a temperature of 40 C. until it is injected. After 

 the injection is finished, place the organ in alcohol for twenty-four hours, to coagulate the 

 gelatine. 



2. Carmine and Gelatine Mass. () Take I oz. of the best gelatine, and make a solution 

 of it as described above. Strain while hot through flannel, and make up the solution to 2 oz. 

 (b) Place I drachm of the best carmine in a mortar, to it add I drachm of liquor ammoniae and 

 2 oz. of water, and leave it for twelve hours (if in a hurry, place the carmine solution near the 

 fire for an hour). Filter, and add about 80 minims of glacial acetic acid, drop by drop, stirring 

 all the while, until the ammonia is completely neutralised. As the odour of the ammonia 

 becomes faint the acid must be added very cautiously. As long as there is free ammonia the 

 fluid is of a dull red, but it becomes of a bright, florid, cherry colour the moment the ammonia 

 is neutralised. Add the florid red carmine fluid (b) to the 2 oz. of gelatine solution (), and keep 

 at a temperature of 40 C. until the mass is required. 



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