216 E. A. ANDREWS. 



they have the form of elongated, not spherical masses that are dis- 

 charged from the anus as the anal sphincter relaxes from time to time. 

 The mucous strand is here broken, the granular aggregates becoming 

 isolated from one another. These balls or pellets move along but 

 slowly and give up the revolving motion seen in the first part of 

 the intestine and in the stomach. 



The time elapsing from the addition of carmine to the water to 

 the discharge of carmine containing pellets may be much less than 

 one hour. 



Though no carmine was seen to enter the diverticnlum, yet after 

 twenty-four hours its walls became pink from the accumulation of 

 fine granules that, in sections, are seen to lie within the epithelial 

 cells of this part of the digestive tract. When the animal is kept 

 long in sand and water to which carmine is added from time to 

 time, this is taken up by many cells on the branchial arches as well 

 as by the cells of the stomach and intestine. Moreover, as demon- 

 strated by Weiss (24) for the European Amphioxus, many cells of 

 the atrium take up the carmine, notably those forming the so-called 

 nephridial ridges and those in the pigmented, dorsal atrial pouches. 

 The nephridia, however, could not be recognized in sections of such 

 individuals. 



After some weeks the pre-oral organ was found darkly stained 

 by the carmine, but not uniformly so : the closed deep lying part 

 contained most of the red in the form of radiating spherules as if 

 in gland cells while the tubular part of the organ opening into 

 the pre-oral chamber had a decidedly yellow color with only diffused 

 red in the free ends of the cells and very few red granules in their 

 basal parts. This appearance of the pre-oral organ suggested at 

 the time that it might have a nephridial function. 



When Bismarck brown is added to the water in which the live 

 animals are kept, they soon became darkly stained. On sectioning, 

 this stain is found to be due to fine spherules in the cells of the 

 epidermis, digestive tract, atrium and testis and also in the cells 

 lining lymph spaces. Here again the method failed in revealing 

 nephridia. 



The vitality of injured specimens is very great; even those cut 

 into two live for some days. In such cases some regeneration of 

 lost parts may be initiated. Thus when the tail was cut off just 



