XIV 



CONTENTS 



XII. The collection of material for bacteriological exami- 



nation, - - 191 



(1) Hair, p. 191. (2) Skin, p. 191. (3) Sputum, p. 191. (4) Blood, 

 p. 192; The collection of serum, p. 195. (5) Pharyngeal exudates, 

 p. 197. (6) Abscesses, p. 197. (7) Aqueous humour, p. 197. (8) 

 Pleural and pulmonary exudates, p. 198. (9) Ascitic fluid, p. 198. 

 (10) Tumours and lymphatic glands, p. 198. (11) Splenic puncture, 

 p. 198 ; Splenectomy, p. 199. (12) Lumbar puncture, p. 199. (13) 

 Milk, p. 201. (14) Urine, p. 201. (15) Stools, p. 202. 



XIII. The bacteriological examination of fluids and tissues. 



Section I. Film preparations, - 203 



(1) Unstained preparations, p. 203. (2) Stained preparations: A. 

 Preparation of films, p. 204. B. Staining methods (i) Simple 

 staining, p. 205. (ii) Differential staining, p. 207. 



Section II. Histological preparations, - 211 



(1) Instruments, p. 211. (2) Freezing methods, p. 212. (3) Paraffin 

 embedding methods, p. 212. (4) Preliminary treatment of sections, 

 p. 215. (5) The staining of sections : A. Simple staining, p. 216. 

 B. Differential staining, p. 217. 



XIV. Immunity. The properties of immune serums. 



Introduction, - - 221 



The mechanism of immunity, p. 222. 



Section I. Prophylactic and therapeutic serums, 223 



Section II. Antitoxins, 224 



Section III. Agglutinins, - 225 



The mechanism of agglutination, p. 226. 



Section IV. Bactericidal properties, - 227 



The mechanism of bacteriolysis, p. 228 ; Hsemolysins, p. 230 ; The 

 preparation of an hsemolytic serum, p. 230 ; The mechanism of 

 haemolysis, p. 231 ; The fixation of the complement, p. 232 ; The 

 technique of the complement-fixation reaction, p. 233. 



Section V. Opsonins, - - 239 



The method of determining the opsonic index, p. 240. 



PART II. THE PATHOGENIC BACTERIA. 



SUB- DIVISION I. THE NON-SPORE-BEARING, GRAM-POSITIVE, 

 NON-ACID-FAST BACILLI. 



XV. Bacillus diphtherias. 



Introduction, - - _ 245 



Section I. Experimental inoculation, - - - 247 



(1) The symptoms and .lesions produced in animals susceptible to 

 infection, p. 247. (2) The influence of other organisms on the clinical 

 course of the disease, p. 249. 



Section II. Morphology, 250 



