CONTENTS xiii 



Section V. The measurement of microscopical objects, - 121 



(1) The experimental determination of the magnification produced by 

 a system of lenses, p. 121. (2) The measurement of objects under the 

 microscope, p. 122. 



Section VI. Dark-ground illumination, - 123 



(1) The application of dark-ground illumination to micro-biology, 

 p. 124. (2) The construction of the dark-ground illuminator, p. 124. 

 (3) The method of using the instrument, p. 125. 



VIII. The microscopical examination of cultures of micro- 



organisms. 



Introduction, - 130 



Section I. The preparation of slides and cover -glasses, - 130 



Section II. The examination of unstained preparations, 131 



(1) The examination of a culture on an ordinary slide, p. 132. (2) 

 Hanging-drop preparations, p. 132. 



Section III. The examination of stained preparations, - - 135 



(1) Staining solutions, p. 137. (2) Simple staining, p. 140. (3) Gram's 

 stain, p. 142. (4) Claudius' method, p. 144. 



IX. The staining of spores, capsules and flagella. The 



study of the motility of bacteria. 



Section I. Spores, - 145 



(1) The examination of unstained preparations, p. 145. (2) The 

 staining of spores, p. 146. 



Section II. The staining of capsules, - 147 



Section III. The staining of flagella, 148 



(1) The staining of flagella in living organisms, p. 148. (2) The 

 staining of flagella in dried preparations, p. 149. 



Section IV. The methods of studying the motility of 



micro-organisms, - - 154 



X. Animal inoculation. 



Section I. The selection of animals for inoculation, 156 



Section II. The keeping of animals, - 157 



Section III. The spontaneous diseases of experimental 



animals, 159 



Section IV. The handling of experimental animals, 160 



Section V. Experimental inoculations, - - 165 



(1) Instruments, p. 165. (2) Preparation of the material for inocula- 

 tion, p. 169. (3) Technique of inoculation, p. 170. 



Section VI. Observations to be made on inoculated animals, 182 



XI. Post mortem examinations. 



Introduction, - 



(1) Instruments, p. 184. (2) Preliminary operations, p. 185. (3) 

 Examination of the external surface of the carcase, p. 185. (4) Exami- 

 nation of the internal organs, p. 185. (5) Removal of tissues for 

 histological examination, p. 188. 



